Development Of Discriminating Monitoring Techniques For Moths Insecticde Resistance In Pectionphora Gossypiella (Lepidoptera: Noctuidae) In Cotton

M. Ali Mater⁽¹⁾ A.A. Khidr⁽¹⁾ H.E. Abd-Elkarim⁽²⁾ E.G. Abo- Elghar⁽³⁾ and A.A. Abd- Elhalim^(1)
(1)Plant Protection Research Institute, Agriculture Research Center, Dokky, Giza, Egypt
⁽²⁾Faculty of Agriculture, Mochtohor, Zagazig University, Egypt
⁽³⁾Faculty of Agriculture, Menoufia University, Egypt

Abstract

Techniques were developed to monitor insecticide resistance in moths of the pink bollworm, Pectinophora gossypiella (Saunders). Adult stage of a susceptible laboratory pink bollworm strain were tested with six insecticides namely Profenofos, Chloropyrifos, Thiodicarb, Carbaryl, Esfenfalerate 5 EC, Esfenfalerate 20 EC, with a glass vial technique to estimate concentration- mortality curves. An approximate Lc99 was selected to discriminate the resistance in three field populations. Tests with pink bollworm moths resulted in 40- 96.7% mortality for different field populations (Kafr-Elshaikh, Menoufia and Beni-suif Governorates ) at early and late season of 2000. A formula [100-(MF/MSx100)] allowed to determine the percentage of resistant insects in the field and simultaneously to correct for partial survival of susceptible insects at the discriminating concentration used. The methods were then tested on field- collected insects during early and late cotton season of 2000. Accuracy of the method was confirmed with another technique for measuring insecticide resistance. These techniques were developed to supplement monitoring data for adult resistance to provide timely and accurate resistance data when the insect is an actual pest, and to provide producers or consultants with a predictive bioassay useful in detecting resistance before any insecticide application.