Dr. Wafaa Choumane

Name: Wafaa Choumane Address: Tishreen University, P.O.Box 2099, Lattakia, Syria Fax: +963-41-216042 (Home) Fax: +963-41-416401 (Work) E-mail1: wafaa627@scs-net.org E-mail2: w.choumane@cgiar.org

• Education:
  1. B . Sc. in Agriculture Sciences: Faculty of Agriculture, Tishreen University, Lattakia, Syria
  2. Diploma of high studies : Section "field crops", Faculty of Agriculture, Tishreen University, Lattakia, Syria
  3. Diploma of deepened studies (D.E.A.) : in Molecular and Cellular Biology, Department of General and Applied Biology, University of Claud Bernard - Lyon I, Lyon, France Title of the experimental D.E.A. thesis: "Molecular characterization of rDNA unit in sunflower (Helianthius annuus)"
  4. Ph.D. in Molecular Biology : Department of General and applied Biology, University of Claud Bernard - Lyon I, Lyon, France. Title of the thesis: "Analysis of genetic variability within the genus Helianthus through the structure of ribosomal genes".
  
   
• Languages:
  1. Arabic (native language)
  2. French
  3. English
  
   
• Profession
  1. Professor, Head of the department of Fundamental Sciences in the Faculty of Agriculture, Tishreen University, Lattakia, Syria.
  2. Consultant in biotechnology at ICARDA in Aleppo, Syria (part time).

• Main activities
  Researchs
  1. 1985 - 1988 - Establishment of restriction rDNA map in Helianthus annuus
     - Isolation, cloning and subcloning of the rDNA unit of sunflower in different vectors
     - Identification of the coding and non-coding regions of rDNA using radioactive rRNA/DNA hybridization
     - Establishment of the Phylogeny, based on the rDNA information, of species of the genus Helianthus
     
  2. 1992 - 1993 - Use of molecular markers in the identification of different varieties of chickpea, through DNA fingerprinting using the digoxygenated oligonucleotide probes.
  3. 1994 - 1996 - Estimation of the genetic distance between and within different genotypes of barley using RFLP and PCR-RAPD protocols.
  4. 1996 - 1998 - Biodiversity study in barley and Cicer genus (chickpeas and wild species). Evaluation of a collection of barley existing at ICARDA and covering the WANA regions. The markers used were PCR-RAPD and STMS.
     For Cicer, the identification and the phylogeny of the wild species was realized with the STMS markers, and the evaluation of the genetic diversity within a chickpea collection was estimated by STMS markers.
  5. 1999 - 2004 - Identification and development of DNA markers linked to the Sitonia resistance in Lens. Molecular techniques used are PCR-RAPD and AFLP. Estimation of the biodiversity in the pine in Syria through the molecular markers PCR-RAPD and AFLP.
     - Identification of dehydrin genes in barley. The techniques used are based on the mRNA to prepare cDNA and study those genes in detail.
     - Mapping of STMS marker linked to drought resistance in barley.
  6. 2004 - 2007 - Genotyping of 3000 accessions of barley collections with SSR and EST-SSR. - Allelic imbalance analysis in barley using SNPs.
  
    Teaching
  Teaching M. Sc and B. Sc. Students in the following disciplines:
  1. Classical Genetic
  2. Molecular Biology
  3. Genetic Engineering
  4. Plant anatomy
  
   
• Supervision and conducting training courses:
  1. Co-supervision training courses in " DNA molecular techniques for germplasm evaluation and crop improvement" in 1994, 1995,1996, 2001at ICARDA, Syria
  2. Co-conductig a training course in " DNA molecular techniques for germplasm evaluation and crop improvement" at INRA, Rabat, Morocco. 19- 30 July 1999
  3. Lectures in the use of DNA markers in biodiversity study. Training on Molecular tools for biodiversity studies. Germplasm Resources Unit (GRU) at ICARDA. Aleppo, Syria. 4-15 July 1999
  4. Lectures in the use of DNA markers (STMS, AFLP) in germplasm evaluation and crop improvement. Training course in Germplasm Program (GP) at ICARDA. September 1999
  5. Conducting a training courses in " DNA molecular techniques for crop improvement" in 2003, in the faculty of Agriculture, Tishreen University, Lattakia, Syria
  6. Conducting a training courses in " DNA molecular techniques for crop improvement" in 2004, in the General Scientific Research Organization , Douma, Damascus, Syria.
  7. Conducting a training courses in " DNA molecular techniques for crop improvement" in 2005 and 2006, ICARDA, Aleppo, Syria.
  
   
• List of Publications:
  Thesis:
  1. Choumane W. (1985) . Structure and evolution of vegetal ribosomal genes. Comparaison with animal and procaryotic rDNA. Review study to obtain the degree of D.E.A. Department of General and applied Biology, University of Claud Bernard - Lyon I, Lyon, France, (In French).
  2. Choumane W. (1985) . Molecular characterization of nuclar rDNA in sunflower (Helianthus annuus). Thesis for the degree of D.E.A. Department of General and applied Biology, University of Claud Bernard - Lyon I, Lyon, France, (In French).
  3. Choumane W. (1988) . : Analysis of genetic variability within the genus Helianthus through the structure of ribosomal genes. Department of General and applied Biology, University of Claud Bernard- Lyon I, Lyon, France, (In French).
  
    Journal Articles (Referred papers) :
  1. Choumane W. and Heizmann P. (1988) . Structure and variability of nuclear ribosomal genes in Helianthus annuus. Theor. Appl. Genet. 76: 481- 489.
      
  2. Choumane W. and Weigand F. (1995). Characterization of Chickpea vareities using DNA fingerprinting. Tishreen University J. (In Arabic)
      
  3. Choumane W., Ashtar S. and Weigand F. (1999) . Genetic diversity in barley evaluated by molecular markers. Al- Manara J. vol 4, 2: 11-22 (in Arabic).
      
  4. Huttel B., Winter P., Weising K., Choumane W., Weigand F. and Kahl G. (1999) . Sequence-tagged microsatellite site markers for chickpea (Cicer arietinu m L.). Genome 42: 210-217, (In English)
      
  5. Ashtar S., Choumane W., Ghazal H., and Baum M. (1999). Evaluation of genetic variability in a barley core collection by using DNA markers. J. of Aleppo University. Vol: 33 (in Arabic).
      
  6. Choumane W., Winter P. Weigand F. and Kahl G. (2000) . Conservation and Variability of Sequences Tagged Microsatellite Sites from Chickpea (Cicer arietinum) within the genus Cicer. Theor Appl Genet . 101: 269-278 , (In English)
      
  7. Choumane W., Ghazal H., Ashtar S. and Baum M. (2000). Genetic diversity in the Syrian barley by using RAPD markers. King Saud University Journal, no. 99. (in Arabic)
      
  8. Choumane W. (2001). The phylogenetic relationships between the annual species of the genus Cicer by using the molecular markers. Tishreen University Journal for Studies and Scientific Research- Agriculture Science Series Vol (In Arabic).
      
  9. Choumane W. Baum M. (2002). Conservation of Sequence Tagged Microsatellite Sites (STMSs) between Cicer and Pisum. Tishreen University Journal for Studies and Scientific Research- Agriculture Science Series Vol. 24, No. 12. (In English)
      
  10. Al-Said W., Choumane W., Ceccarelli S., Baum M. and Lababidi S. (2003). Identification of dehydrin genes in barley. R. J. of Aleppo Univ., Basic sciencse serires, No. 39 . (In English).
       
  11. Moualla M., Ghazal H., Choumane W. and Elwaoui H. (2004). Genetic relationship and yield characterization of local chickpea populations. Tishreen University Journal for Studies and Scientific Research- Agriculture Science Series. Vol 26, No 1 , (In Arabic).
       
  12. Choumane W., Winter P., Baum M. and Kahl G. (2004). Conservation of microsatellite flanking sequences in different taxa of Leguminosae. Euphytica, 138, 239-245. (In English)
       
  13. Choumane W., P. van Breugel, T.O.M. Bazuin, M. Baum, G.W. Ayad and W. Amaral. (2004). Genetic Diversity of Pinus brutia in Syria detected by molecular markers. Forest genetics, Vol. 11 (2): 87-101, (In English).
       
  14. Hamwieh, A. Udupa, S.M., Choumane, W., Sarker, A., Dreyer, F., Jung, C., Baum M. (2005). A genetic linkage map of lentil based on microsatellite and AFLP markers and localization of fusarium vascular wilt resistance. Theor. Appl. Genet. . Theor Appl Genet, 110: 669-677, (In English).
       
  15. El Amir G. , Sabbouh M., Hassan I., Choumane W., and Baum M. (2006). Genotyping and assessing genetic diversity in Pisum collection using AFLP markers. Submitted to Tishreen University J. (In English)
       
  16. Kholani M., Choumane W. and Moualla M., and Baum M. (2007) . Genetic diversity in a Yemen barley Germplasm using Microsatelitte markers. (submitted to Tishreen University J.)(In Arabic)
  
    Conference, symposium Papers and posters:
  1. Choumane w. and Heizmann p. and Berville A. (1986) . Structure and variations of the nuclear ribosomal genes in the genus Helianthus. Poster presentation. International Symposium on Plant Molecular Biology. Strasbourg, France. July 1-4
      
  2. Choumane w. and Heizmann P. (1987) . Structure and variations of nuclear DNA in the genus Helianthus. Comparaison between H.annuus and H. Tuberosus. Poster presentation. Session D. Organisation and Expression of nuclear and cytoplasmic Genomes. First symposium of young researchers of the French of vegetal physiology. University of Paul Sabatier, Toulouse, France. June 18 - 19
      
  3. Choumane w. (1992). Identification and comparaison of rDNA of sixty species of the genus of Helianthus. Proceedings of the 32nd science week. Damas, Syrie, 7-13 Novembre
      
  4. Choumane w. (1993). Use of biotechnology tools in estimation the genetic distance within the genus Helianthus. Proceedings of the Second Arab Conference on Perspectives of Modern Biotechnology. Amman, Jordan, April 24-28
      
  5. Choumane w., Weigand F. and Udupa S. (1994) . Application of DNA marker for varietal identification and estimation of intraspecific variations in chickpea populations. In: Proceedings of Symposium of Application of DNA Fingerprinting to Crop Improvement: Molecular marker assisted breeding of chickpea. ICARDA, Alep, Syrie, April 11-14
      
  6. Choumane w. and Weigand F. (1994) . Determination of the DNA finferprints of chickpeas and its agronomical applications. Proceedings of the 34th Science Week. Damascus, Syria, , Novembre 5-7.
      
  7. Choumane w. and Weigand F. (1996) . Estimation of the genetic distance between different genotypes of barley using the techniques of RFLP and PCR-RAPD. Proceedings of the 36th Science Week. Aleppo, Syria, November 2-11.
      
  8. Choumane w., Ashtar S. Valkoun J. and Weigand F. (1997). The use of DNA markers in the study of biodiversity in barley. The Third International Triticeae Symposium, IPGRI, May 4-8. Aleppo, Syria.
      
  9. Choumane W., Ashtar S. and Weigand F. (1997) . Biodiversity in barley evaluated by molecular markers. "Biotechnology: presence and future perspective" Symposium. April 7-8 , Amman, Jordan
      
  10. Winter P., Huttel B., Pfaff T., Wolf H., Weigand F., Udupa S., Choumane W., Saxena M., Muehlbauer F., Kahl G. (1998). Molecular markers and genetic diversity in chickpea (Cicer arietinum). Page: 200 in 3rd European conference on grain legumes: Opportunities for high quality, healthy and added value crops to meet European demands. Proceeding, AEP/UNIP/PGRO, 14-19 November, Valladolid, Spain.
       
  11. Ashtar S., Choumane w., Ghazal H. and Baum M. (1998). STMS markers to evaluate the genetic diversity in a barley core collection. Cairo, Egypt. 24-28 December , 1998
       
  12. Choumane Wafaa and Baum Michael (1999). Selected biotechnologies for crop improvement in WANA countries. International Conference: "Technology Transfer in Biotechnologies as a Catalyst for National Development". October 4-6, Beirut, Lebanon
       
  13. Choumane w. and Baum M. (2000). The use of RAPD markers for characterization of annual species of the genus Cicer. Cairo, Egypt 20-22/11/2000
       
  14. Choumane w. and Baum M. (2001). Development of DNA markers linked to the Sitonis critinus H. resistance in Lens. Poster, The international conference of biotechnology in the arid regions, in Kuwait.
       
  15. Choumane W, and Baum M. (2001). Molecular markers linked to Sitonis critinus H. resistance in Lens. The 41st Science week, conference of biotechnology. Damascus, 3-5 November 2001.
       
  16. Lababidi S., Choumane w., Ceccarelli S., Grando S., Saiid W., and Baum M. (2002). Identification of Dehydrin genes in barley. The international conference of biotechnology , Egypt.
       
  17. Choumane w. (2003). The genetically modified plants, Production and detection methods. The 43 Science week, conference on genetically modified plants, Homs, 2-- -2-- November 2003.
       
  18. Lababidi S., Choumane w., Ceccarelli S., Grando S., Saiid W., and Baum M. (2004). Allelic variation of Dehydrin genes in barley. 9th International Barley Genetics Symposium , 20 - 26 June, 2004, Brno, Czech Republic
       
  19. Hamwieh, A., W. Choumane, S.M. Udupa , F. Dreyer , C. Jung , M. Baum. (2004). Development of Microsatellite markers for the genus Lens. Plant & Animal Genomes XII Conference, January 10-14, 2004, San Diego, CA.
       
  20. Choumane w., Baum M., Russell J. , Grando S., and Ceccarelli S. (2005). Using DNA markers for the identification of drought tolerant accessions in barley. Improving Water-Use Efficiency in MEDiterranean agriculture: what limits the adoption of new technologies? WUEMED Rome, September 29-30 .
       
  21. Valkoun J., Choumane W., Jing Z., Hong C., Zhens L. Grando S., Ceccarelli S and Baum M Baum M., Russell J (2005). .Poster in InterDrought-II, An International aconference on Itegrated Approches to Improve Crop Production Under Drought Prone Environments. Italy, September 2005
       
  22. W. Choumane, Z. Jing, J. Valkoun, M. Baum. (2005). Developing a composite germplasm set of barley and its characterization with genomic and genic SSR markers. Poster. Rome, Italy, 28-30 September, 2005
       
  23. Choumane w. and M. Baum (2006). DNA Markers for characterization and evaluation of genetic diversity in barley. Lecture. Workshop in genetic engineering in Qatar, June, 6-7.
       
  24. Choumane w. , Jing Z., J. Valkoun J., Hong C., Zhens L. and Baum M. (2006). Assessing genetic diversity in a composite germplasm set of barley and the development of a core collection. Poster presented in Sao Paolo, Brazil, 13-18 September 2006.
       
  25. Choumane w. (2006). Role of Genetic Engineering in the Sustainable Agriculture. Conference on Role of Biotechnology in the sustainable Agriculture. Lecture: Role of Genetic Engineering in the sustainable Agriculture and the difficulties. The 46 Science week, conference of biotechnology. lattakia, 20-23- November 2006.