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Expression of the Insecticidal Protein Gene Cry 1c of Bacillus thuringiensis in the
Plant-Colonizing Nitrogen Fixing Bacteria
- Nahed A. A. Ibrahim (1) and Omar, M. N. A.(2)
- (1)Department of Microbial Molecular Biology, Agricultural Genetic Engineering Research Institute, ARC, Giza
- (2)Soil & Water Research Institute, ARC, Giza
- The Third International Conference onIPM Role in Integrated Crop management and Impacts on Environment and Agricultural Products. 26-29 November 2005, Giza, Egypt.
Abstract
The NMO10 bacterial isolate was isolated from the rhizosphere of cotton plants. This isolate showed high potentiality to fix nitrogen and high ability to colonize the phyllosphere of cotton plants. Based on this criterion, the plasmid pHTNC3 harboring cry1C gene (Nahed, 2001) was used to transform the NMO10. The transformed bacterial isolate (tNMO10) proved dual action both as bio-control of the Lepidopteron insect Spodoptera littoralis and as bio-fertilizer. The presence of expressed 135 kDa protein of the CryIC in the protein pattern of the tNMO10, the microscopic examination of the bipyramidal crystals that characterized the CryIC protein and the immuonoblot analysis indicated efficient expression of cryIC gene in the heterologous host NMO10. Bioassays against the neonate larvae of the cotton leaf worm S. littoralis revealed that the protein preparations from the tNMO10 were toxic
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| New Publications |
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The Arab Scientists in Cooperation with The High Institute of Marine Research. Publish all the researches abstracts, which presented in The Regional Workshop on Marine Sciences and Natural Resources, Tishreen University, Lattakia, Syria, 25-26 May 2004.
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| New Publications |
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The Arab Scientists in Cooperation with The Egyptian Society for Biological Control of Pests . Publish all the researches abstracts, which presented in The First Arab Conference of Applied Biological Pest Control, Cairo, Egypt, 5-7 April 2004.
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| Conferences, Fez |
The First International Symposium on Environnement protection for development Biotechnological aspects. Fez, December
15 – 17, 2004.
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| Conferences, Syria |
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The Second Syrian-Egyptian conference in the Agriculture and Food.
Agriculture Faculty, Al-Baath university, Homs, Syria. 25-28 April, 05
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| Conference,Yemen |
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Current Status of Plant Protection in Yemen and Future Strategy.
Faculty of Agriculture, Sana'a University, Sana'a, yemen. December 18-20/2004
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| Conferences Canada |
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The 9th International Conference of the Orthopterists’ Society
Canada, on August 14 19, 2005
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| Conferences, Egypt |
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The First Arab Conference on Applications of Biological Control of Pests in Arab Countries
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| Workshop,Lebanon |
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New Workshop
Middle-East Academy for Medicine of Ageing
First Annual Middle-East Age & Aging Meeting
8-11 April, 2004
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| Publications |
Arab Society for Plant Protection (ASPP) in Cooperation with The Arab Scientists
Publish all the researches abstracts, which presented in the Eighth Arab Congress of
Plant Protection, held in Faculty of Agriculture, Omar Al-Mukhtar University, El-beida
City, Libya, October 12-16,2003
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