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A genetic linkage map of Lenssp. based on microsatellite and AFLP markers
and the localization of fusarium vascular wilt resistance
- A. Hamwieh, S. M. Udupa, W. Choumane, A. Sarker, F. Dreyer, C. Jung, M. Baum
- Theor Appl Genet, 110: 669-677
Abstract
Microsatellites have currently become the markers of choice for molecular mapping and markerassisted selection for key traits such as disease resistance in many crop species. We report here on the mapping of microsatellites which had been identified from a genomic library of lentil (Lens culinaris Medik.). The majority of microsatellite-bearing clones contained imperfect dinucleotide repeats. A total of 41 microsatellite and 45 amplified fragment length polymorphism (AFLP) markers were mapped on 86 recombinant inbred lines derived from the cross ILL 5588 · L 692-16-1(s), which had been previously used for the construction of a random amplified polymorphic DNA and AFLP linkage map. Since ILL 5588 was resistant to fusarium vascular wilt caused by the fungus Fusarium oxysporum Shlecht. Emend. Snyder & Hansen f.sp. lentis Vasud. & Srini., the recombinant inbreds were segregating for this character. The resulting map contained 283 markers covering about 751 cM, with an average marker distance of 2.6 cM. The fusarium vascular wilt resistance was localized on linkage group 6, and this resistance gene was flanked by microsatellite marker SSR59-2B and AFLP marker p17m30710 at distances of 8.0 cM and 3.5 cM, respectively. These markers are the most closely linked ones known to date for this agronomically important Fw gene. Using the information obtained in this investigation, the development and mapping of microsatellite markers in the existing map of lentil could be substantially increased, thereby providing the possibility for the future localization of various loci of agronomic interest.
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| New Publications |
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The Arab Scientists in Cooperation with The High Institute of Marine Research. Publish all the researches abstracts, which presented in The Regional Workshop on Marine Sciences and Natural Resources, Tishreen University, Lattakia, Syria, 25-26 May 2004.
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| New Publications |
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The Arab Scientists in Cooperation with The Egyptian Society for Biological Control of Pests . Publish all the researches abstracts, which presented in The First Arab Conference of Applied Biological Pest Control, Cairo, Egypt, 5-7 April 2004.
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| Conferences, Fez |
The First International Symposium on Environnement protection for development Biotechnological aspects. Fez, December
15 – 17, 2004.
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| Conferences, Syria |
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The Second Syrian-Egyptian conference in the Agriculture and Food.
Agriculture Faculty, Al-Baath university, Homs, Syria. 25-28 April, 05
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| Conference,Yemen |
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Current Status of Plant Protection in Yemen and Future Strategy.
Faculty of Agriculture, Sana'a University, Sana'a, yemen. December 18-20/2004
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| Conferences Canada |
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The 9th International Conference of the Orthopterists’ Society
Canada, on August 14 19, 2005
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| Conferences, Egypt |
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The First Arab Conference on Applications of Biological Control of Pests in Arab Countries
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| Workshop,Lebanon |
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New Workshop
Middle-East Academy for Medicine of Ageing
First Annual Middle-East Age & Aging Meeting
8-11 April, 2004
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| Publications |
Arab Society for Plant Protection (ASPP) in Cooperation with The Arab Scientists
Publish all the researches abstracts, which presented in the Eighth Arab Congress of
Plant Protection, held in Faculty of Agriculture, Omar Al-Mukhtar University, El-beida
City, Libya, October 12-16,2003
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