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Initiation And Characterization Of A New Cell Line From Spodoptera Littoralis (New Sl)
- Omaima Khamiss1.2; E. H. Alexandra1;
Ammar El.Desouky1 and Abol-Ela. S1
- 1 Center of virology, Faculty of Agriculture-IRD, Cairo University, Egypt.
- 2 Genetic Engineering and Biotechnology Institute- Mnoufieya University,
Sadate city, Mnoufieya, Egypt
Abstract
A new cell line was established and cloned from pupal ovaries of Egyptian cotton leaf worm Spodoptera littoralis and characterized at the morphological level, growth, and karyotyping level. The chromosomal number was compared to a reference cell line Sf9 and to eggs of the same insect. Cells were spherical at the beginning but some of them turned to be spindle shaped, maximum cell population found to be 4.8x106 at the logarithmic phase, cell doubling time was 24hr. Chromosomes were typical to those described from other species of Lepidoptera. Chromosomes number was counted on 57 metaphase for the new cell line (new Sl) and it ranged between 4-203 Chromosomes where as 23% of counted metaphases contained 6 chromosomes only. As for 58 metaphases were prepared from eggs, the counted chromosomal number ranged between 4-24 on 57 metaphases and only one metaphase had 46 chromosomes, but 20% of metaphases showed 8 chromosomes. The obtained results for eggs and new Sl was different from Sf9 which showed chromosomal number range from 61- 263 in 68 metaphase,; from which 30% was ranged from 103-120.
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| New Publications |
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The Arab Scientists in Cooperation with The High Institute of Marine Research. Publish all the researches abstracts, which presented in The Regional Workshop on Marine Sciences and Natural Resources, Tishreen University, Lattakia, Syria, 25-26 May 2004.
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The Arab Scientists in Cooperation with The Egyptian Society for Biological Control of Pests . Publish all the researches abstracts, which presented in The First Arab Conference of Applied Biological Pest Control, Cairo, Egypt, 5-7 April 2004.
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The First International Symposium on Environnement protection for development Biotechnological aspects. Fez, December
15 – 17, 2004.
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The Second Syrian-Egyptian conference in the Agriculture and Food.
Agriculture Faculty, Al-Baath university, Homs, Syria. 25-28 April, 05
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Current Status of Plant Protection in Yemen and Future Strategy.
Faculty of Agriculture, Sana'a University, Sana'a, yemen. December 18-20/2004
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| Conferences Canada |
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The 9th International Conference of the Orthopterists’ Society
Canada, on August 14 19, 2005
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| Conferences, Egypt |
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The First Arab Conference on Applications of Biological Control of Pests in Arab Countries
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| Workshop,Lebanon |
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New Workshop
Middle-East Academy for Medicine of Ageing
First Annual Middle-East Age & Aging Meeting
8-11 April, 2004
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| Publications |
Arab Society for Plant Protection (ASPP) in Cooperation with The Arab Scientists
Publish all the researches abstracts, which presented in the Eighth Arab Congress of
Plant Protection, held in Faculty of Agriculture, Omar Al-Mukhtar University, El-beida
City, Libya, October 12-16,2003
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