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Virogenesis In New Established Spodoptera Littoralis Cell Line(Sl 96), Adapted At 19 C Permissive For Spli.Granulovirus, Phop.Granulovirus, And Spli.Nucleopolyhedrovirus
- Omaima Khamiss
- Center of virology, Faculty of Agriculture-IRD, Cairo University, Egypt. Genetic Engineering and Biotechnology Institute- Mnoufieya University, Sadat city, Monufieya, Egypt.
Abstract
A complete replication of the Spodoptera littoralis granulovirus (SpliGV), nucleopolyherovirus (SpliNPV), and Phthoremea operculella granulovirus (PhopGV) was obtained in vitroby both viral infection and DNA transfection in the S.littoralis (Sl96) cell line which was established and completely characterized from ovariouls tissues of the cotton leaf worm, Spodoptera littoralis. SpliGV and PhopGV multiplied significantly during several passages in Sl96 cells at 19°C. Cells were infected and kept at 19°C for the first 4 hours post -infection. Then all the infected cells were incubated at 27°C till end of the experiment (20 days). The viral multiplication proceeded at the same rate. Comparison of SpliGV progenies multiplied either in vivo or in vitro, using electron microscopy and restriction profile analysis, showed their identity. Viral infections were detected also by specific nucleic probes.
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| New Publications |
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The Arab Scientists in Cooperation with The High Institute of Marine Research. Publish all the researches abstracts, which presented in The Regional Workshop on Marine Sciences and Natural Resources, Tishreen University, Lattakia, Syria, 25-26 May 2004.
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| New Publications |
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The Arab Scientists in Cooperation with The Egyptian Society for Biological Control of Pests . Publish all the researches abstracts, which presented in The First Arab Conference of Applied Biological Pest Control, Cairo, Egypt, 5-7 April 2004.
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| Conferences, Fez |
The First International Symposium on Environnement protection for development Biotechnological aspects. Fez, December
15 – 17, 2004.
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| Conferences, Syria |
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The Second Syrian-Egyptian conference in the Agriculture and Food.
Agriculture Faculty, Al-Baath university, Homs, Syria. 25-28 April, 05
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| Conference,Yemen |
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Current Status of Plant Protection in Yemen and Future Strategy.
Faculty of Agriculture, Sana'a University, Sana'a, yemen. December 18-20/2004
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| Conferences Canada |
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The 9th International Conference of the Orthopterists’ Society
Canada, on August 14 19, 2005
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| Conferences, Egypt |
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The First Arab Conference on Applications of Biological Control of Pests in Arab Countries
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| Workshop,Lebanon |
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New Workshop
Middle-East Academy for Medicine of Ageing
First Annual Middle-East Age & Aging Meeting
8-11 April, 2004
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| Publications |
Arab Society for Plant Protection (ASPP) in Cooperation with The Arab Scientists
Publish all the researches abstracts, which presented in the Eighth Arab Congress of
Plant Protection, held in Faculty of Agriculture, Omar Al-Mukhtar University, El-beida
City, Libya, October 12-16,2003
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