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Positive Effect Of Fluorescent Brightener 28 And Neemazal T/S On The Activity Of Agrotis Sgetum Granulovirus Tested Against The Black Cutworm Agrotis Ipsilon Hb. Larvae (Lepidoptera: Noctuidae)

  • KHATTAB M. MAGDA2, S. EL-SALAMOUNY1, M.A.K. EL-SHEIKH1, A. AMIN2 and S. ELNAGAR1
  • 1 Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, 12613- Giza, Egypt.
  • 2 Cutworm Unit, Plant Protection Institute, Agricultural Research Center, Ministry of Agriculture, Giza, Egypt

Abstract


Fluorescent brightener 28 and Neemazal T/S (Neem) were used at different concentrations to increase the efficacy of AgseGV tested against Agrotis ipsilon neonate larvae. Fluorescent brightener tested alone, did not result in any larval mortality whereas, the rate of larval mortality due to virus increased with increasing concentrations of the additive alone. The rate of enhancement of virus activity was 1096.678 fold at 0.1% concentration. The calculated LT50 value was decreased from 11.47 to 4.66 days at the concentration of 3.85x107 capsule/ml diet when combined with 0.1% F. brightener. Neemazal T/S was added to three different concentrations (1,5 and 10, ppm) of AgseGV and tested against A. ipsilon neonate larvae. The results demonstrated that the neem oil at 10 ppm poorly enhanced the activity of AgseGV by reducing the LC50 value from 3.59x107 to 7.11x106 capsule/ ml diet (potency= 5.057 fold). The calculated LT50 value was decreased from14.13 days to 13.14, 10.56 and 7.2 days for the tested neemazal concentrations, respectively. In conclusion, the combination of each additive with the baculovirus, AgseGV may improve the activity of virus formulations.


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