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Low Virulence Variability Of Four Egyptian Spodoptera Littoralis (Boisd.) Nucleopolyhedrovirus Isolates
- Luc BRUN2, Said EL-SALAMOUNY1, Wael KAMAL2 and Said ABOL ELA1
- 1 Department of Economic Entomology and pesticides, Faculty of Agriculture, Cairo University, Giza, Egypt
- 2 Centre of Virology, IRD- Faculty of Agriculture, Cairo University, P.O. Box 26, Giza, Egypt.
Abstract
The virulence of four Spodoptera littoralis (Boisd.) nucleopolyhedro virus isolates collected from three regions: Giza (two isolates 1&2), Alexandria and Fayoum, were tested againstS. littoralisneonate larvae. The source of S. littoralis colony was collected from Giza region and maintained on an artificial media. The bioassay data showed no big variations in the obtained median lethal concentration (LC50) with a value of 1.6 x 105, 5.6x10 4, 2.5x104 and 1.7x104 PIB's/ml diet, for the Giza 1, Giza 2, Alexandria and Fayoum viral isolates, respectively. At the same time, the Fayoum viral isolate was used to compare the susceptibility of three S. littoralis larval strains collected from Baharia Oasis, Fayoum Oasis and Giza regions. The LC50 values were 1.6x105, 5.4x104 and 7.3x104 PIB's/ml for the above mentioned three strains, respectively. The observed low variability of the LC50 among virus strains or insect strains was in the normal variability range under the bioassay conditions. The genomic variability among the four viral isolates is still under investigation.
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