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The Use OfBacillus Thuringiensis Var. Kurstaki In Protecting Stored Bee Wax Combs And Wax Foundations Agianst The Greater Wax Moth, Galleria MellonellaL.
- Omar, Naglaa,A.M1, El-Husseini,M.M2 and El-Bishry,M.H.1
- 1 Agriculture Research Center, Plant Protection Research Institute, Dokki, Giza, Egypt.
- 2 Biological Control Research Laboratories, Faculty of Agriculture, Cairo University, Giza, Egypt.
Abstract
Toxicological study using the commercial formulation Dipel 2X based on the entomopathogenic bacterium, Bacillus thuringiensis var. kurstaki in the concentrations of 2, 4, 6, 8 and 10 g/ 100g diet were carried out versus larvae (L3) of the greater wax moth, Galleria mellonella L.. The calculated LC50 reached 4.784g/100g diet. The LC100 (9.568g/100ml water) was used to treat the wax combs and foundation sheets by the spraying technique that followed by artificial infestation with eggs of the greater wax moth (ca 200 egg/comb or foundation sheet) and stored in wooden boxes at 26-27 °C and 60-70% R.H. Weight of stored Dipel 2X-treated and untreated bee wax material was estimated each 2 months for one year. Infestation was completely reduced (zero%) in B.t.var. kurstaki treated wax combs and foundation sheets, while untreated combs (control) lost 26, 60,90,98, and 100% of their wax content after 2, 4, 6, 8 and 10 months post treatment, respectively. Also, respective losses of 28, 66, 91, 98 and 100% were recorded for untreated wax foundation sheets at the same intervals.
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