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Biological Control Of Soybean Damping-Off Caused By Sclerotium Rolfsii Using Pseudomonas fluorescens And Pseudomonas putida

  • Ahmed M. Hassanien, (1), and Gamal A.A. Mekhemar. (2).
    (1) Plant Pathology Research Institute, Agric. Research Center, Giza, Egypt
    E-mail ahmedhassanein48@yahoo.com
    (2) Soils, Water and Environment Research Institute, Agric. Research Center, Giza, Egypt

Abstract


Two biocontrol agents Pseudomonas fluorescens and Pseudomonas putida were evaluated for controlling of soybean damping-off caused by Sclerotium rolfsii under laboratory and greenhouse conditions. The effect of both biocontrol agents on nodulation and growth of soybean was also investigated. Data showed that Pseudomonas fluorescens and Pseudomonas putida caused significant reduction in mycelial radial growth of Sclerotium rolfsii on king’s B medium only. The results obtained showed that Pseudomonas fluorescens and Pseudomonas putida significantly reduced pre- and post-emergence damping-off caused by S. rolfsii and increased plants survival individually or combined with B. japonicum (USDA 110 + 3407). The maximum protection of soybean plants was achieved by seed inoculation with Pseudomonas fluorescens combined with B. japonicum. Co-inoculation with Pseudomonas fluorescens and B. japonicum gave the best results in terms of number, dry weight of nodules, shoots, roots and their N-content. Generally, it was clear that the best results were obtained in case of co-inoculation with the three biocontrol agents which reduced damping-off and enhanced nodulation status and growth of soybean plants. .


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