|
In-Vitro And In-Vivo Studies On The Use Of Bacillus Subtilis To
Control Fusarium Oxysporum F.Sp. Lentis, The Causal Organism Of
Lentil Vascular Wilt
- Said El-Hassan , Simon Gowen (1) and Bassam
Bayaa (2)
(1) School of Agriculture, Policy and
Development, Reading University, Eraley Gate, P.O. Box 236, Reading RG6 6AT,
Berkshire, UK.
E-mail: s.el-hassan@reading.ac.uk
(2)
) ICARDA, P.O. Box 5466, Aleppo, Syria.
Abstract
Lentil (Lens culinaris Medikus) is an important legume crop in Syria.
Vascular wilt, caused by Fusarium oxysporum f.sp. lentis, is one
of the most important diseases affecting lentil worldwide. Understanding the
dynamics of the pathogen and its bio-control agent in the soil is an important
factor in determining the success of biological control. The antagonistic
activity of the biocontrol agent (Bacillus subtilis) evaluated as the
percent reduction of fungal (Fusarium oxysporum f. sp. Lentis)
growth due to the antifungal metabolites produced by the antagonistic bacterium
was studied in both in-vitro and in-vivo bioassays. Results from both assays
revealed that B. subtilis and its filtrate had a strong inhibitory effect
on spore germination and mycelial growth of F. oxysporum f. sp.
lentis. In a growth room study, a population of Bacillus subtilis applied to
15-days-old susceptible lentil (ILL 4605) seedlings, at 5 ml (8X106
cfu/ml-1)/plant, rapidly increased up to 107 (cfu/g soil)
in 10 days following its application and slightly decreased to 10 6
(cfu/g soil) in 5 weeks after its application. However, in a glasshouse
experiment, the population density of B. subtilis in the soil was greater
than 109 (cfu/g) after 2 weeks of drenching the bacterium suspension
either alone or in the presence of the pathogen. This was accompanied by a
decrease in wilt incidence of about 70% during 73 days from planting.
|
|
| New Publications |
|
The Arab Scientists in Cooperation with The High Institute of Marine Research. Publish all the researches abstracts, which presented in The Regional Workshop on Marine Sciences and Natural Resources, Tishreen University, Lattakia, Syria, 25-26 May 2004.
|
| New Publications |
|
The Arab Scientists in Cooperation with The Egyptian Society for Biological Control of Pests . Publish all the researches abstracts, which presented in The First Arab Conference of Applied Biological Pest Control, Cairo, Egypt, 5-7 April 2004.
|
| Conferences, Fez |
The First International Symposium on Environnement protection for development Biotechnological aspects. Fez, December
15 – 17, 2004.
|
| Conferences, Syria |
|
The Second Syrian-Egyptian conference in the Agriculture and Food.
Agriculture Faculty, Al-Baath university, Homs, Syria. 25-28 April, 05
|
| Conference,Yemen |
|
Current Status of Plant Protection in Yemen and Future Strategy.
Faculty of Agriculture, Sana'a University, Sana'a, yemen. December 18-20/2004
|
| Conferences Canada |
|
The 9th International Conference of the Orthopterists’ Society
Canada, on August 14 19, 2005
|
| Conferences, Egypt |
|
The First Arab Conference on Applications of Biological Control of Pests in Arab Countries
|
| Workshop,Lebanon |
|
New Workshop
Middle-East Academy for Medicine of Ageing
First Annual Middle-East Age & Aging Meeting
8-11 April, 2004
|
| Publications |
Arab Society for Plant Protection (ASPP) in Cooperation with The Arab Scientists
Publish all the researches abstracts, which presented in the Eighth Arab Congress of
Plant Protection, held in Faculty of Agriculture, Omar Al-Mukhtar University, El-beida
City, Libya, October 12-16,2003
|
|
