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Production Of Insect Resistant Potato By ýAgrobacterium Mediated
Transformation With Cry1ac ýGene From Bacillus Thuringiensisýý
A.J. Khan, A.M. Al-Saadi, A.M. Al-Subhiý
College of
Agricultural and Marine Sciences, Sultan Qaboos University, P.O. Box ýý34,
AlKhod 123, Sultanate of Omaný
E-mail: Saad2000@squ.edu.com
Abstract
In Oman, potato cultivation has increased in recent years as consumer ýdemand
for potato products has risen. Tuber moth (Phthorimaea ýoperculella) is a
serious constraint to increase the potato production in Oman. ýThe insecticidal
proteins, (-endotoxins) are specifically lethal to Lepidopteran ýinsects. An
attempt is being made to transform a potato cultivar with Bacillus
ýthuringiensis gene which codes for an insecticidal protein. In order to
express ýendotoxin in transgenic potato, a cry1Ac coding sequences from B.
ýthuringiensis has been constructed into Agrobacterium vector (pRD400) with
ýdouble 35S AMV promoter suitable for dicot transformation using kanamycin
ýresistance gene as marker. Transgenic callus of potato has been grown on
modified ýMS medium containing 300 mg/L carbenicillin and 150 mg/L kanamycin.
The ýintegration of cry1Ac gene into potato genome has been verified by PCR
ýamplification.ý
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The Arab Scientists in Cooperation with The High Institute of Marine Research. Publish all the researches abstracts, which presented in The Regional Workshop on Marine Sciences and Natural Resources, Tishreen University, Lattakia, Syria, 25-26 May 2004.
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The Arab Scientists in Cooperation with The Egyptian Society for Biological Control of Pests . Publish all the researches abstracts, which presented in The First Arab Conference of Applied Biological Pest Control, Cairo, Egypt, 5-7 April 2004.
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The First International Symposium on Environnement protection for development Biotechnological aspects. Fez, December
15 – 17, 2004.
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The Second Syrian-Egyptian conference in the Agriculture and Food.
Agriculture Faculty, Al-Baath university, Homs, Syria. 25-28 April, 05
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Current Status of Plant Protection in Yemen and Future Strategy.
Faculty of Agriculture, Sana'a University, Sana'a, yemen. December 18-20/2004
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The 9th International Conference of the Orthopterists’ Society
Canada, on August 14 19, 2005
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The First Arab Conference on Applications of Biological Control of Pests in Arab Countries
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New Workshop
Middle-East Academy for Medicine of Ageing
First Annual Middle-East Age & Aging Meeting
8-11 April, 2004
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| Publications |
Arab Society for Plant Protection (ASPP) in Cooperation with The Arab Scientists
Publish all the researches abstracts, which presented in the Eighth Arab Congress of
Plant Protection, held in Faculty of Agriculture, Omar Al-Mukhtar University, El-beida
City, Libya, October 12-16,2003
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