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Study Of Organophosphate (Op) Resistance Mechanism In Culex Pipiens Mosquitoes ( Diptera:Culicidae)

Naima Laias(1) and Amanda Callaghan(2)
(1)Department of Zoology, Faculty of Science, University of Alfateh, P.O. Box 13106, Tripoli, Libya
(2)Division of Zoology, School of Animal and Microbial Sciences, the University of Reading, Whiteknights, P.O. Box 228, Reading, RG6 6AJ, UK

Abstract


Culex pipiens mosquitoes are vectors of diseases such as Filariases in tropics and viruses in temprate and tropics areas. These vectors are controlled by chemicals including organophosphates insecticides. Since 1970 the effectiveness of the chemical control has been reduced by the development of resistance that has been linked to the increase in carboxylesterase activity. Previous studies had shown that the esterase B1 gene was activated 500 times in OP Culex Tem-R strain. A B1 DNA probe was used for the study of the structure and organisation of B1 esterase gene amplification. This will help understanding the mechanism of the B1 amplification, which is the aim of this study.



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