Ovicidal And Electrophoretic Patterns Of Eggs Soluble Protein Of Spodoptera Littoralis As Affected By Insecticides

Z.H. Zidan⁽¹⁾ M.I. Abdel Mageed⁽¹⁾ S.A. Emara⁽²⁾ A.E. Bayoumi⁽¹⁾ and F.I. El-Sweerk^(2)
(1)Department Plant Protection, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
E-mail: zidan42@hotmail.com
⁽²⁾Plant Protection Research Institute, Agriculture Research Center, Dokki, Giza, Egypt

Abstract

The ovicidal activity of eight different compounds relates to different insecticidal functional groups were investigated by dipping technique against Spodoptera littoralis eggs of two ages, i.e. one-and three-day old egg - masses. The LC50 values (for one-day old egg-mass) were descendingly arranged as follows: 0.5, 1.4, 2.5, 3.3, 16.3, 24.2, 133.3 and 188.2 ppm for deltamethrin, flufenoxuron, Bioneem, cyanophos, Nat-l, Garlic gard, pyriproxyfen and Vertemic, respectively. As for the three- day old egg - masses, deltamethrin, flufenoxuron and cyanophos were noticeably more effective than the other compounds with LC50 values 0.6, 0.7 and 1.9 ppm, respectively, while the other compounds tested showed moderate effect. On the other hand, the soluble protein of both untreated and treated egg-masses of 1 and 3-days old with five tested materials, i.e. pyriproxyfen, Bioneem, Garlic Gard, Nat-1 and deltamthrin were separated electrophoretically using SDS polyacrylamide gel electrophoresis (SDS-PAGE). Results indicated that both number of the separated soluble protein bands and their corresponding molecular weights were affected by the treatments with the compounds tested. Usually, the compounds tested caused an increase in bands number of the separated soluble proteins.