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Biochemical Evaluation Of The Placental Transfer Of A Single Oral Dose Of
Chlorpyrifos-Methyl In Pregnant Rats
A.E. Bayoumi(1) Z.H. Zidan(1) E.N. El-
Khatib(2) and M. Reem Ziada(2)
(1)Department of Plant Protection, Faculty of Agriculture,
Ain-Shams University, Shubra El- Kheima, Cairo, Egypt
E-mail: zidan42@hotmail.com
(2)Central
Agriculture Pesticides Laboratory (CAPL), Dokki, Giza, Egypt
Abstract
The placental transfer of certain pesticides in female rats was studied. The
pesticides tested included chlorpyrifos-methyl and methomyl as representative
insecticides to the organophosphorus and carbamates chemical class,
respectively. The placental transfer of the selected compounds was studied
thorough their effects on certain biomarkers, i.e. acetylcholinesterase, total
glutathione content, glutathione S-transferase and ATP-ase in various organs of
the pregnant rats and their corresponding foetuses. To perform such study single
oral dose of 1/10 and 1/30 of LD50 of the pesticides tested were administered on
day 18th of gestation to pregnant Spergue-Dawly rats. The treated rats were
killed after 10 min and 0.5, 1, 3, 6, 12, 24, and 48 hr to obtain the desired
organs and foetuses. Results obtained indicated that the two compounds were
transferred through placental route and produced a significant decrease in the
AChE activity of brain and serum of pregnant dams and their corresponding foetus
brain AChE activity was time and dose-dependent. Also, the data showed a
significant increase in liver total glutathione content. Conversely significant
decrease in the placental GSH was recorded. The two doses of chlorpyrifos-methyl
and methomyl tested caused a significant decrease in foetus liver GSH. The liver
GSH content significantly decreased at 12 hr after lactated mothers treatment
with 1/10 of LD50 of chlorpyrifos-methyl, but the lower dose level produced an
alteration effect in relation to time after treatment. In case of glutathione
S-transferases, the pesticides tested caused a significant alteration in the
placental and serum GST activity of the pregnant dams. Administration of
chlorpyrifos-methyl and methomyl to pregnant dams' produced a significant
decrease in brain ATP-ases activity. Foetuses brain ATP-ases activities were not
affected significantly by the treatment with the compounds tested.
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