Effects Of 10.5 Ghz Microwave Radiation On Some Biological Activities Of Two Species Of Staphylococcus Bacteria

Saba A.S.H. Al-Sultan⁽¹⁾ aTayma N.Al-Ghulami ⁽²⁾ and Ramzia H.A. Rahman ^(1)
(1) Section of Medical Biology, Department of Anatomy, College of Medicine, University of Mosul, Iraq
⁽²⁾ Section of Medical Physics, Department of Physiology, College of Medicin, University of Mosul, Iraq.

Abstract

In this work, 18 hours pure BHI broth cultures of Staphylococcus aureus (coagulase positive) and Staphylococcus saprophyticus (coagulase negative) were subjected to microwave radiation at 10.5 GHz of 15 mW for 15, 30, 45, 60, 75, 90, 105 and 120 minutes, respectively. The aim was to know whether microwave radiations have an effect on some vital biological activities including sensitivity test and some routine diagnostic tests such as Catalase, Oxidase, and Slide coagulase tests. Results indicate that the most important effect of microwave radiation was on the sensitivity test of coagulase negative S. saprophyticus (CNS) more than its effect on coagulase positive S. aureus (CPS). The (CNS) bacteria changed from being sensitive as in the control sample to resistant at 15.75 min for Amicacin (30 ƒÝg) and at 45 min for ceftriaxone (30 ƒÝg) and at 75 min for Gentamicin (30 ƒÝg). In contrast, these bacteria were more sensitive to some antibiotics due to microwave exposure. Most important is its transformation from being resistant to Cefoxitin (30 ƒÝg) to sensitive at 75 min exposure time. Also, Catalase test of both types of bacteria has changed from being positive to negative at exposure times of (30 and120 min.) of (CPS) bacteria and 120 min of (CNS) bacteria. Another effect of microwave radiation is the change in the results of Slide coagulase test from positive of (CPS) bacteria to negative at exposure time of 30 min. Finally, the microwave radiation had an effect on Oxidase test for both types of bacteria.