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Conservation and variability of sequence-tagged microsatellite sites
(STMSs) from chickpea (Cicer arietinum L.) within the genus Cicer
By: W. Choumane (1,3), P. Winter (2), F. Weigand (1) and G.Kahl (2), 2000.
(1): Centre for Agricultural Research in the Dry Areas (ICARDA), P.O. Box 5466, Alepo, Syria
(2): Plant Molecular Biology, Biocentre, University of Frankfurt/Main, D-60439 Frankfurt, Germany
(3): Faculty of Agriculture, University of Tishreen, Lattakia, Syria.
Theor Appl Genet. 101: 269-278 (in English).
Abstract
The conservation of 90 microsatellite-flanking sequences from chickpea in 39 accessions of annual and 1 accession of perennial species of the genus Cirer was investigated. All of the primer sequences successfully amplified microsatellites in related species indicating the conservation of microsatellite-f1anking sequences in chickpea's relatives. However, the degree of conservation of the primer sites varied between species depending on their known phylogenetic relationship to chickpea ranging from 92.2% in C. reticulatum, chickpea's closest relative and potential ancestor, down to 50% for C. cuneatum. A phvlogenetic tree revealed that the other members of its crossability group were more closely related to the perennial C. anatolicum than to other annual species of the genus. Considerable variation in size and number of amplification products between and within Species was observed. Sequence analysis of highly divergent amplification products proved that variation is either due to large differences in the number of microsatellite repeats or to the amplification of a locus unrelated to the one amplified from chickpea. Sequence information and bootstrap using PAUP suggested hat STMSs derived from chickpea may be efficiently and reliably used for synteny studies in chickpea's crossability group, including C.anatolicum . However care should be taken when applying these markers to other species of the genus. Considering the data presented here and the known historical record, the age of section Monocicer, including chickpea, is estimated to be about 100,000 years.
Key words: Cicer species, Microsatellites, Zero-allele, Phylogenetic tree, Evolution.
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