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Conservation of Sequence Tagged Microsatellite Sites (STMSs) between Cicer and Pisum
By: Wafaa Choumane (1), Michael Baum(2)
(1) Basic Science Department, Faculty of Agriculture, Tishreen University, Lattakia, Syria
(2) International Center for Agricultural Research in the Dry Areas, (ICARDA), P.O. Box 5466, Aleppo, Syria
Abstract
sequence-tagged microsatellite sites (STMS) are proved to be valuable descriptors of plant genetic variation, but developing markers for each taxon is very costly. The applicability of STMS markers may be increased if they can be amplified across taxa. To test if microsatellite markers designed for one genus are informative in related genera, the presence and efficiency of intergeneric primer binding sites were tested in the Fabaceae family. A total of 70 STMS derived from chickpea were used to amplify loci in the dry pea pisum.
The percentage of chickpea primers producing amplification products in dry pea was 67.1%. Hybridization of selected amplification products from the two genera to digoxigenated labelled oligonucleotide (TAA)5 used as a probe showed that some loci were very well conserved between the tested genera whereas others were not. STMS derived from chickpea could be used for the detection of variability within and between other genera of Fabaceae, but care should be taken if homologous loci are compared.
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| New Publications |
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The Arab Scientists in Cooperation with The High Institute of Marine Research. Publish all the researches abstracts, which presented in The Regional Workshop on Marine Sciences and Natural Resources, Tishreen University, Lattakia, Syria, 25-26 May 2004.
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| New Publications |
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The Arab Scientists in Cooperation with The Egyptian Society for Biological Control of Pests . Publish all the researches abstracts, which presented in The First Arab Conference of Applied Biological Pest Control, Cairo, Egypt, 5-7 April 2004.
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| Conferences, Fez |
The First International Symposium on Environnement protection for development Biotechnological aspects. Fez, December
15 – 17, 2004.
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| Conferences, Syria |
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The Second Syrian-Egyptian conference in the Agriculture and Food.
Agriculture Faculty, Al-Baath university, Homs, Syria. 25-28 April, 05
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| Conference,Yemen |
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Current Status of Plant Protection in Yemen and Future Strategy.
Faculty of Agriculture, Sana'a University, Sana'a, yemen. December 18-20/2004
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| Conferences Canada |
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The 9th International Conference of the Orthopterists’ Society
Canada, on August 14 19, 2005
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| Conferences, Egypt |
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The First Arab Conference on Applications of Biological Control of Pests in Arab Countries
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| Workshop,Lebanon |
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New Workshop
Middle-East Academy for Medicine of Ageing
First Annual Middle-East Age & Aging Meeting
8-11 April, 2004
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| Publications |
Arab Society for Plant Protection (ASPP) in Cooperation with The Arab Scientists
Publish all the researches abstracts, which presented in the Eighth Arab Congress of
Plant Protection, held in Faculty of Agriculture, Omar Al-Mukhtar University, El-beida
City, Libya, October 12-16,2003
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